PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
نویسندگان
چکیده
منابع مشابه
Preparation of lipide extracts from brain tissue.
For the preparation of lipide extracts from tissues, the method of Bloor (I), either in its original form or with slight modifications, has been a standard procedure. This method consists in extracting the tissue with a mixture of ethyl alcohol and ether. Since the extract obtained is known to contain non-lipide contaminants, it is usually taken to dryness and the residue extracted with a solve...
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The initial procedure in the isolation of an protein, a protein complex, or a subcellular organelle is the preparation of an extract that contains the required component in a soluble form. Indeed, when undertaking a proteomic study, the production of a suitable cellular extract is essential. Further isolation of subcellular fractions depends on the ability to rupture the animal tissues in such ...
متن کاملPreparation of Lipid Extracts from Tissues
A. Introduction B. Storage of Tissues and Preliminary Treatments prior to Extraction C. Extraction of Tissues with Solvents D. Removal of Non-Lipid Contaminants from Extracts E. Artefacts of Extraction Procedures F. Practical Extraction Procedures 1. Some general comments 2. The "Folch" procedure 3. The Bligh and Dyer method 4. Extraction of plant tissues G. Some Special Cases 1. Acyl-coenzyme ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1951
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)55987-1